![]() An internal reference standard was incorporated into the assay, serving as a normalization point for cystatin C quantification. Cystatin C, a cysteine proteinase inhibitor and a potential marker for several pathological processes, was used as a target analyte. Presented here is a mass spectrometric immunoassay method for targeted quantitative proteomics analysis of protein modifications. Mass spectrometry, coupled to immunoaffinity separations, can provide an efficient mean for simultaneous detection and quantification of protein variants. While sandwich immunoassays are routinely used to determine protein concentrations, they are oblivious to protein variants that may serve as biomarkers with better sensitivity and specificity than their wild-type proteins. Modified proteins also play an important role in biological processes. Post-translational modifications and genetic variations give rise to protein variants that significantly increase the complexity of the human proteome. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |